Airplane and airport bathrooms harbor "extremely high and concerning levels" of bacteria, including human feces, according to research cited by experts. The study, conducted by Sanctuary Bathrooms, found that public toilets in both environments contained over 32,000 colony-forming units (CFUs) of fecal streptococci, a bacterium commonly found in human waste. James Roberts, director of Sanctuary Bathrooms, described the findings as a "significant cause for concern."

To mitigate the risk of germ exposure, experts suggest several hygiene practices for travelers. Packing antibacterial wipes is recommended for cleaning high-touch surfaces such as toilet flush buttons and taps. These wipes are an inexpensive way to reduce contamination and are available for purchase in multi-packs. Hand sanitizer is also deemed an essential item, especially if soap dispensers in airplane restrooms are empty or non-functional.

Travelers are advised to pack extra toilet paper or specific toilet wipes, but caution is urged against flushing wipes, even those labeled as flushable, as they can clog aircraft plumbing systems. The practice of walking barefoot or in socks inside airplane lavatories is discouraged. A former commercial flight attendant noted that the liquid on lavatory floors can be more than just water and may include urine or spilled drinks. Infectious disease specialist Dr. Dahlia Philips warned that walking barefoot exposes individuals to bacteria, viruses, and fungi. Opting for disposable slippers or slip-on shoes with hard soles can provide better foot protection.

For those needing to carry personal items into the restroom, a small travel bag is suggested to avoid potential contact with surfaces. To freshen up during flights, travelers have found travel-sized mouthwash useful, with many bottles fitting within the 100ml liquid restrictions for carry-on luggage. Disposable toothbrushes that require no water, such as Colgate Wisps, are also recommended as a way to maintain cleanliness and avoid germs.