The Lawn Tennis Association (LTA) has implemented a complete ban on transgender women participating in any female tennis matches, encompassing even internal club competitions. This decision follows the introduction of new transgender eligibility rules by the Women's Tennis Association (WTA), which mandates genetic testing to determine a player's eligibility.

Previously, transgender women were prohibited from county, regional, and inter-club matches. However, individual clubs retained the discretion to allow transgender women to compete in their internal events. This flexibility has now been removed.

The LTA's updated regulations align with recent guidance from the Equalities and Human Rights Commission, which asserts that "sex is binary." This move by the LTA is seen as a direct consequence of this interpretation.

The WTA's revised policy requires players to undergo sex screening for the SRY gene, a genetic marker typically associated with male development. Passing this test, conducted via a cheek swab, blood test, or saliva sample, is now a prerequisite for competing on the female-only WTA Tour. Further medical assessments may be required for players who initially test positive, before they are cleared to compete against cisgender female athletes. Refusal to undergo testing could lead to disciplinary action.

World No. 1 Aryna Sabalenka has expressed her support for these new rules, emphasizing the importance of fairness in the sport. "It's obvious that, biologically, men are much stronger than women, so it wouldn't seem fair to me for a woman to compete against a biological man," Sabalenka stated. She indicated her willingness to undergo testing herself.

The LTA's original guidance from 2024 acknowledged that tennis and padel are "gender-affected sports," noting that the average male possesses physical advantages, including longer limbs and greater cardiovascular capacity, which can impact gameplay.

Similar eligibility testing measures have been adopted by other sports organizations, including World Boxing and World Athletics. The International Olympic Committee (IOC) has also confirmed plans for sex testing athletes in preparation for the 2028 Los Angeles Olympic Games.